INTERNATIONAL JOURNAL OF PURE & APPLIED BIOSCIENCE
ISSN : 2320-7051
International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (340) Last page : (346 )
Article doi: : http://dx.doi.org/10.18782/2320-6820
Validation of DNA Markers Linked to MYMV Resistance in Mungbean (Vigna radiata (L). R. Wilczek)
Prakash Patel*, Kaushal Modha, Chintan Kapadia, Gopal Vadodariya and Ritesh Patel
Department Of Genetics And Plant Breeding, N. M. College Of Agriculture, Navsari Agricultural University, Navsari – 396 450, (Gujarat) India
*Corresponding Author E-mail: firstname.lastname@example.org
Received: 24.07.2018 | Revised: 18.08.2018 | Accepted: 27.08.2018
Mungbean yellow mosaic virus (MYMV) is the most important disease of mungbean causing significant yield loss. Present investigation was carried out to identify molecular marker linked with MYMV resistance gene by investigating F5 recombinant inbred lines (RILs) developed from the cross between Meha (resistant) and GM-4 (susceptible) at hot spot with GM-4 as an infector. The RILs segregated in 1R:1S ratio in the field screening indicating that the MYMV resistance gene inherited as a monogenic trait and MYMV resistance is under monogenic control. Out of 124 primers studied including RAPD, SSR, SCAR and RGA primers, only one primer RGA pair 1F-CG/RGA 1R amplified a single 445 bp band in the genotype Meha (resistant parent) and MYMV resistant bulk which was absent in GM-4 (susceptible parent) and MYMV susceptible bulk. Same amplicon was detected in individual samples of F5 RILs constituting the MYMV resistant bulk indicating that this RGA was linked to MYMV resistance. This marker can be used in screening mungbean genotypes for resistance to MYMV disease.
Key words: Recombinant Inbred Lines, Mungbean Yellow Mosaic Virus, Resistant Gene Analogue.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Patel, P., Modha, K., Kapadia, C., Vadodariya, G. and Patel, R., Validation of DNA Markers Linked to MYMV Resistance in Mungbean (Vigna radiata (L). R. Wilczek), Int. J. Pure App. Biosci. 6(4): 340-346 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6820