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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (613) Last page : (624)
Article doi: :

Microbial Hazard Analysis of Branded and Retail Milk Sold in Parbhani City of Maharashtra State, India

Joshi S. R. Deshmukh V. V.*, Waghmare R. N. and  Yeotikar P. V.

Department of Veterinary Public Health & Epidemiology, College of Veterinary & Animal Sciences,
Parbhani, Maharashtra Animal & Fishery Sciences, Nagpur
*Corresponding Author E-mail:
Received: 31.05.2018 | Revised: 28.06.2018   | Accepted: 8.07.2018  



An experiment was conducted to study the microbial hazard analysis of milk sold in Parbhani city. A total of 306 milk sample comprising of 34 milk  samples from each of five pasteurized milk brands (A, B, C, D and E) and 136 raw milk samples were analyzed during the study. A significantly high (p<0.05) Total Viable Count (TVC) was observed amongst all the sources of milk samples. The raw milk sample were having high TVC (14.99 ± 0.79) than pasteurized milk (14.54) samples. Amongst pasteurized milk samples TVC was high in brand C than other brands. The E.coli counts were significantly high (p<0.05) amongst all the sources. However, E.coli counts of raw milk were the highest (15.05 ± 0.19). A total of 69 E.coli isolates were obtained from 198 milk samples. The identification of these isolates was made by biochemical characterization. A total of 16 E.coli isolates were characterized by using PCR. The isolates have been submitted to Veterinary Type Culture Center (NRCE), Hissar (Haryana) for further typing and maintenance at Repository. Streptococci were also isolated from all the sources. However, a non significant effect of different sources upon streptococcal counts was seen. A total of 70 streptococcal isolations were confirmed with 35.35 percent positivity. An attempt to isolate Salmonella was done but organisms could not be isolated. A total of six clostridial isolates were obtained amongst 198 milk samples. Biochemical characterization has been done. The overall prevalence of 3.03 percent was observed.

Key words: Microbial hazard, Analysis, Branded and Retail milk, Parbhani city.

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Cite this article: Joshi, S.R., Deshmukh, V.V., Waghmare, R.N. and Yeotikar, P.V., Microbial Hazard Analysis of Branded and Retail Milk Sold in Parbhani City of Maharashtra State, India, Int. J. Pure App. Biosci.6(4): 613-624 (2018). doi: