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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (738) Last page : (741)
Article doi: :

Characterization of the Supernatants of the Growth of Potent Rhizobacteria

Shreeja* A. N. and Chattannavar S. N.

*Part of the M. Sc. (Agri.) Thesis submitted by the first author to the Department of Plant Pathology,
College of Agriculture, Vijayapur, University of Agricultural Sciences, Dharwad- 580005, Karnataka, India
*Corresponding Author E-mail:
Received: 14.08.2018  |  Revised: 23.09.2018   |  Accepted: 30.09.2018  



A common screen for plant antimicrobial compounds consists of separating plant extracts by paper or thin-layer chromatography (PC or TLC). The effectiveness of this screening method, known as bioautography, depends on both the quality of the chromatographic separation. Screening for their plant growth-promoting properties, and metabolic characterization. In pigeon pea associated rhizobacteria displayed a large metabolic activity. And the antibiotics produced by PGPR include: butyrolactones, zwittermycin A kanosamine, oligomycin A, oomycin A, phenazine-1-carboxylic acid, pyoluteorin, pyrrolnitrin, viscosinamide, xanthobaccin, and 2,4-diacetyl phloroglucinol. 2,4-diacetyl phloroglucinol (2,4-DAPG) is one of the most efficient antibiotics in the control of plant pathogens and can be produced by various strains of Pseudomonas, one of the most common bacterial species of the rhizosphere. It has a wide spectrum of properties in that it is antifungal. The TLC methods apply to many types of plant extracts and other bacterial species (aerobic or anaerobic), as well as fungi, can be used as test organisms if culture conditions are modified to fit the growth requirements of the species.

Key words: Antimicrobial compounds, Rhizobacteria, Thin layer chromatography, Metabolic characterization, Screening

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Cite this article: Shreeja, A.N. and Chattannavar, S.N., Characterization of the Supernatants of the Growth of Potent Rhizobacteria, Int. J. Pure App. Biosci. 6(5): 738-741 (2018). doi: