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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (797) Last page : (802)
Article doi: :

Assessment of Safe Dose of Clove Oil for Using as Anesthetics in Aquaculture Operations

V. P. Saini1, Alok D. Kamble2, M. L. Ojha2 and Surnar Sharad Raosaheb2*

1Aquaculture Research and Seed Unit, Directorate of Research,
2Department of Aquaculture, College of Fisheries,
Maharana Pratap University of Agriculture and Technology, Udaipur – 313001, India
*Corresponding Author E-mail:
Received: 13.08.2018  |  Revised: 21.09.2018   |  Accepted: 29.09.2018  



The safe concentration of clove oil for aquaculture operations was determined by conducting bioassay tests in static waters. For this purpose five doses (0.04, 0.05, 0.06, 0.07, and 0.08 ppm) were applied in glass aquaria of 28 liters capacity. Applying these concentrations of clove oil for 24 and 48 hours, fish, C. carpio survival rate was compared with control. The estimated LC50 values for 24 and 48 hrs. were 0.062 and 0.057 ppm respectively. The second order polynomial regression analysis between fish survival and clove oil concentration proved that a dose of 0.02 ppm is safe for use in fish seed transportation.

Key words: Anesthetics, Aquaculture, Toxicity, Clove oil, Fry, Transportation, Static water.

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Cite this article: Saini, V.P., Kamble, A.D., Ojha, M.L. and Raosaheb, S.S., Assessment of Safe Dose of Clove Oil for Using as Anesthetics in Aquaculture Operations, Int. J. Pure App. Biosci.6(5): 797-802 (2018). doi: