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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (921) Last page : (929)
Article doi: : http://dx.doi.org/10.18782/2320-6889

Cytotoxicity Assessment of Nanoparticulate Doxycycline (DH LNP) and Rifampicin against Human Macrophage (U 937) Cell Line

Prashant P. Mhase¹, Anilkumar S. Bannalikar², Vikas V Karande³*, S. V. Londhe⁴ and P. Devarajan⁵

^1,2,4Department of Microbiology, ³Department of Pharmacology,
Bombay Veterinary College, Parel, Mumbai -400012, India
⁵Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai- 400019
*Corresponding Author E-mail: drvikas111081@gmail.com
Received: 17.08.2018  |  Revised: 26.09.2018   |  Accepted: 3.10.2018  

 ABSTRACT

The present study was conducted in order to investigate the cytotoxic effect of Doxycycline (DH), Rifampicin (RIF) and their nanoparticulate formulations (DH LNP and NP RIF) in human U937 macrophages. These drugs are commonly used for the treatment of brucellosis. The DH was found cytotoxic for the cells at 10 µg /ml whereas at concentrations ranging from 0.5 - 5 µg /ml it proved non-toxic. The DH LNP was found to be safe for macrophages between the concentrations of 0.5 - 2 µg/ml however it proved toxic at 5 µg/ml and above. The RIF was non-cytotoxic between the range of 0.5 to 10 µg /mL whereas it was cytotoxic at 20 µg/mL & above. The NP RIF proved safe for macrophages up to a concentration of 5 µg/mL whereas, at 10 µg/mL and above it proved cytotoxic.  The comparison of results revealed a greater cytotoxicity of nanoparticulate formulations of drugs than the plain drugs.  The combination of DH + RIF was non-cytotoxic between the range of 0.5 to 20 µg /mL, however, it proved cytotoxic at 25 µg/mL & above. Similarly, the combination of nanoparticulate drugs DH LNP + NP RIF was found to be safe for macrophages up to the concentration of 20 µg/ml while it proved cytotoxic at concentration of 25 µg/ml and above. Both the methods employed for assessment of cytotoxicity i.e. trypan blue dye exclusion technique and MTT assay were found equally effective. 

Key words: Cytotoxicity, Doxycycline, Human 937, Nanoparticle, Rifampin,

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Mhase, P.P., Bannalikar, A.K.S., Karande, V.V., Londhe, S.V., and Devarajan, P., Cytotoxicity Assessment of Nanoparticulate Doxycycline (DH LNP) and Rifampicin against Human Macrophage (U 937) Cell Line, Int. J. Pure App. Biosci.6(5): 921-929 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6889