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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (1147) Last page : (1156)
Article doi: :

Identification of Beneficial Endophytes Which Helps in Suppressing Black Rot Disease in Cauliflower

Amrita Kanchan1*, Dinesh Singh2, Archana Tiwari1 and Kundan Kumar1

1Noida International University, 2Division of Plant Pathology, IARI, PUSA New Delhi
1Noida International University, Noida  (India)
*Corresponding Author E-mail:
Received: 28.09.2018  |  Revised: 22.10.2018   |  Accepted: 30.10.2018  



Black rot is a destructive disease of cauliflower through out the world caused by Xanthomonas campestris pv. campestris (Pammel) Dowson. The black rot pathogen is seed-borne and spreads in the vascular system of the leaf and stem. In the present study endophytic microorganisms that colonize the internal tissues of plants enhance agricultural production through plant growth promoting mechanisms. Considering the enormous potential of the endophytic bacteria, a research programme was framed to study the role of these bacteria in plant growth promotion of crop plants belonging to vegetables. The endophytic bacteria were isolated from root and shoot  of cauliflower plants. Forty one out of the eighty eight bacterial isolates selected were Gram positive belonging to Bacillus sp. while forty seven were Gram negative belonging to
Citrobacter, Acinetobacter, Pseudomonas.
Root and shoot associated endophytic bacteria were isolated, selected and characterized for their biotechnological potential of growth promotion and biocontrol of plant pathogenic bacteria. Eighty eight strains were isolated and subjected to chemical tests, and the maximum  producer of  HCN as well as ammonia were PSBnR5 and PSBkS10. The 16S rRNA sequence of isolates PSBkS10 and PSBnR5 have been analyzed and submitted in GenBank with accession numbers MH257751 (Pseudomonas fluorescens) and MH257752 (Bacillus subtilis), respectively. Phylogenetic tree of 16S rRNA of both isolates was constructed using the neighbor- joining (NJ) algorithm with bootstrap analysis for 1000 replicates

Key words: Endophytes, Biocontrol agent, Bacillus subtilis, Pseudomonas fluroscens,

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Cite this article: Kanchan, A., Singh, D., Tiwari, A. and Kumar, K., Identification of Beneficial Endophytes Which Helps in Suppressing Black Rot Disease in Cauliflower, Int. J. Pure App. Biosci.6(5): 1147-1156 (2018). doi: