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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (150) Last page : (160)
Article doi: : http://dx.doi.org/10.18782/2320-6880

Genetic Variability Analysis of Sorghum (Sorghum bicolor L. Moench) Genotypes by Using SSR Markers

Zinzala Sumita, Davda B. K., Modha K. G., Vadodariya G. D. and Rathod Snehal

Department of Genetics and Plant Breeding,
N. M. College of Agriculture, NAU, Navsari-396450, Gujarat
*Corresponding Author E-mail: sumitazinzala@gmail.com
Received: 14.07.2018 | Revised: 21.08.2018 | Accepted: 28.08.2018

 

 ABSTRACT

DNA markers are being increasingly utilized in cultivar development, seed production quality control, measurement of genetic diversity for conservation, management, varietal identity and to tackle issues related to intellectual property rights. The aim of this study was to assess genetic variability among 24 genotypes of sorghum by simple sequence repeats (SSRs) technique using 22 primers set. The total number of amplified PCR bands was 460, of which 436 (94.78%) were polymorphic displaying PIC values ranging from 0.11 to 0.77 with an average of 0.52. Genetic coefficient of similarity among the genotypes ranged from 0.25 to 1.00. The use of simple sequence repeats (SSRs) for variety profiling can provide high discrimination with excellent reproducibility at low cost.

Key word: Sorghum, variability, SSR marker. 

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Sumita, Z., Davda, B.K., Modha, K.G., Vadodariya, G.D. and Snehal, R., Genetic Variability Analysis of Sorghum (Sorghum bicolor L. Moench) Genotypes by Using SSR Markers, Int. J. Pure App. Biosci.6(5): 150-160 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6880