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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (161) Last page : (166)
Article doi: : http://dx.doi.org/10.18782/2320-6529

DNA Fingerprinting of CUMS 17 (Suprava): A Newly Developed Variety of Sesame

Maini Bhattacharjee¹*, S. H. Prakash, Souvik Roy², Tamina Begum1 and Tapash Dasgupta²

¹Department of Genetics and Plant Breeding, Institute of Agricultural Science,
Calcutta University, Kolkata 700019, India
²School of Agriculture and Rural development, Ramakrishna Mission Vivekananda University,
Narendrapur, Kolkata 700103
*Corresponding Author E-mail: mainibhattacharjee02@gmail.com
Received: 27.05.2018  |  Revised: 24.06.2018   |  Accepted: 4.07.2018  

 

 ABSTRACT

The variety CUMS 17 (Suprava) has recently been developed in sesame by Genetics and Plant Breeding department, University of Calcutta. The variety is recommended by central varietal release committee in 2018. The variety CUMS 17 along with two check varieties namely GT 10 and Savitri were analysed by 21 SSR primers. Fourteen polymorphic SSR primers were finally used for DNA fingerprinting. The SSR primers produced a total of 46 alleles with an average number of 3.28 alleles with amplicon size ranging from 100 to 600 bp. Alleles were assigned with numeric numbers in the order of increasing size of amplicon that produced an allelic barcode that denotes the unique identification of the newly developed variety of sesame.

Key words: DNA Fingerprinting, Microsatellite markers, Variety identification, Sesame.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Bhattacharjee, M., Prakash, S.H., Roy, S., Begum, T. and Dasgupta, T., DNA Fingerprinting of CUMS 17 (Suprava): A Newly Developed Variety of Sesame, Int. J. Pure App. Biosci.6(5): 161-166 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6529