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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (549) Last page : (555 )
Article doi: : http://dx.doi.org/10.18782/2320-6859

Molecular Characterization and Identification of Biocontrol Isolates of Azotobacter sp. Antagonistic to Fusarium solani in Chilli Crop

Swapna¹*, K. Tamil Vendan¹, Mahadevaswamy¹, D. S. Aswathanarayana², B. Kisan³ and R. C. Gundappagol¹

¹Department of Agricultural Microbiology, ²Department of Plant Pathology,
³Department of Plant Biotechnology, College of Agriculture, University of Agricultural Sciences, Raichur
*Corresponding Author E-mail: swapnav1357@gmail.com
Received: 10.07.2018 | Revised: 17.08.2018 | Accepted: 23.08.2018  

 ABSTRACT

Chilli is one of the most important commercial crop in India and it needs proper care for getting higher yields since it is susceptible to many of the soil diseases. Majority of the farmers will go for chemical sprays which are not at all advisable; conversely we have lot of biocontrol agents which will effectively suppress the activity of the pathogens under different agro-climatic conditions. In search of new and native biocontrol agents, we have investigated 75 Azotobacter isolates from the rhizospheric soils obtained from the chilli grown areas in Raichur, Kalaburgi, Bellary and Koppal region. All the isolates were evaluated for their biological deterrent activity against Fusarium solani, and we found four Azotobacter isolates were effectively inhibited the mycelial growth of F. solani under in vitro conditions. Maximum per cent inhibition of 60.25 was observed with AZT-R7 isolate followed by AZT-Y2 (59.17 %), AZT-J1 (57.77 %) and   AZT-G4 (51.47 %). Further these isolates were characterized by using ten RAPD primers, in Jacob coefficient analysis Azotobacter isolates formed two main clusters viz, AZT-R7 and AZT-Y2; AZT-G4 and AZT-J1. Both the clusters were related by 27 per cent only. However, within the clusters, the AZT-R7 and AZT-Y2 were related by 44 per cent whereas, AZT-G4 and AZT-J1 by 45 per cent. These potential isolates can be recommended for the commercial production as a biocontrol agent. 

Key words: Azotobacter, Biocontrol, Chilli, F. solani, RAPD etc.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Swapna, Tamil Vendan, K., Mahadevaswamy, Aswathanarayana, D.S., Kisan, B. and Gundappagol, R.C., Molecular Characterization and Identification of Biocontrol Isolates of Azotobacter sp. Antagonistic to Fusarium solani in Chilli Crop, Int. J. Pure App. Biosci.6(5): 549-555 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6859