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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (584) Last page : (589)
Article doi: : http://dx.doi.org/10.18782/2320-6638

Influence of Epidermal Growth Factor in the In vitro Development of Bovine Preimplantation Embryos

Sadekar Gautami Vinayak, Timiri Varadarajan. Meenambigai*, Angappan. Mangalagowri, Devarajan Reena and Kumanan. Vijayarani

Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Vepery, Chennai – 600 007
*Corresponding Author E-mail: meena_dharmaraj@yahoo.co.in
Received: 20.06.2018  |  Revised: 27.07.2018   |  Accepted: 5.08.2018

 ABSTRACT

Epidermal growth factor (EGF) plays a crucial role in early embryogenesis. We investigated whether in vitro culture medium supplementation with epidermal growth factor (EGF) has effects in the development of bovine oocytes and embryos. Cumulus–oocyte complexes (COCs) were matured in TCM 199 medium (control) and in TCM 199 medium with EGF at concentrations of 10 - 50 ng/ml. After maturation for 44–46 h, Metaphase II oocytes were assessed based on the degree of cumulus expansion and extrusion of first polar body. Homogenously expanded cumulus oocyte complexes (COCs)were fertilized, and the presumptive zygotes were cultured in the same groups. Then, 2, 4, 8 cells and morulae were collected for the analysis of quality. A concentration of 30 ng/ml of EGF in maturation medium increased MII oocyte numbers and did not affect oocyte maturation. Medium Supplementation with remaining concentration of EGF resulted in lower maturation of oocytes and in control embryos. EGF treatment also increased the cleavage rate and morula percentages respectively.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Vinayak, S.G., Meenambigai, T.V., Mangalagowri, A., Reena, D. and Vijayarani, K., Influence of Epidermal Growth Factor in the In vitro Development of Bovine Preimplantation Embryos, Int. J. Pure App. Biosci.6(5): 584-589 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6638