INTERNATIONAL JOURNAL OF PURE & APPLIED BIOSCIENCE
ISSN : 2320-7051
International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 6
First page : (814) Last page : (822)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7215
Molecular Variability Study among Bipolaris sorokiniana (Sacc) Shoem Isolates-Incitant of Spot Blotch of Wheat
Ashwini R.* and P.V. Patil
Department of Plant Pathology, College of Agriculture, Dharwad
University of Agricultural Sciences, Dharwad-580 005, Karnataka India
*Corresponding Author E-mail: firstname.lastname@example.org
Received: 7.11.2018 | Revised: 20.12.2018 | Accepted: 27.12.2018
Spot blotch caused by Bipolaris sorokiniana is an important disease of wheat. Assesment of molecular variability among 14 isolates of Bipolaris sorokiniana using ten SSR markers revealed high degree of genetic variability. A total of 40 polymorphic bands were produced out of 50 amplicons resulted from 10 different markers. The primer BS035 produced the highest number of amplicons (Twelve) followed by BS074 with nine amplicons. The lowest of one marker level amplicon was produced by BS065.Based on simple matching coefficient a genetic similarity matrix was constructed to assess the genetic relatedness among isolates of B. sorokiniana. The similarity coefficients were ranged from 0.53 to 0.98 representing less than 53 per cent genetic variability among 14 isolates. Further, the dendrogram constructed by UPGMA from the pooled data clearly showed 2 major clusters viz., A and B at a similarity co efficient of 0.53. Cluster A comprised of eight isolates viz., Bsm1, Bsg, Bsm2, Bsa, Bsj, Bsu, Bsd1and Bsn at a similarity co efficient of 0.64 while, cluster B comprised of six isolates viz., Bsd2, Bsp1 Bsdbl1, Bsdbl2, Bsb and Bsp2 at a similarity co efficient of 0.66.
Key words: Bipolaris sorokiniana, Amplicons, Cluster, Similarity coefficient.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Ashwini, R. and Patil, P. V., Molecular Variability Study among Bipolaris sorokiniana (Sacc) Shoem Isolates-Incitant of Spot Blotch of Wheat, Int. J. Pure App. Biosci.6(6): 814-822 (2018). doi: http://dx.doi.org/10.18782/2320-7051.7215