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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 6
First page : (635) Last page : (643)
Article doi: :

Phytochemical Characterization of Bioactive Compound from the Ensete superbum SeedPowder

R. Nirmal Kumar1, P. Muthukumaran2*, K. Suresh Kumar3 and   R. Karthikeyen4

1Assistant Professor, Agricultural Engineering, Sri Shakthi Institute of Engineering and Technology, Sri Shakthi Nagar, L & T by Pass, Chinniyampalayam Post, Coimbatore, Tamil Nadu 641062, India
2Faculty in Science, Institute of Virtual & Distance Learning (IVDL), DMI St. Eugene University,
P. O. Box: 330081, Great North Road, Chibombo, Zambia
3,4 Department of Food Safety and Quality Testing Laboratory, Indian Institute of Food Processing Technology (Formerly Indian Institute of Crop Processing Technology) Ministry of Food Processing Industries,
Government of India    Pudukkottai Road, Thanjavur - 613 005
*Corresponding Author E-mail:
Received: 18.11.2018  |  Revised: 23.12.2018   |  Accepted: 27.12.2018  



The bioactive components Ensete superbum Seed Powder has been evaluated using GCMS, HPLC, FTIR and HNMR. The chemical compositions of the extract of Ensete superbum Seed Powder was investigated by Gas Chromatography–Mass Spectrometry and GC/MS techniques. The analysis of extract of Ensete superbum Seed Powder revealed that the existence of Eugenol (39.51) n-Hexadecanoic acid (21.97), 9-Eicosyne (5.18), 3-Decanynoic acid(1.87), 1-Tetradecyne(5.30), 7-Methyl-Z-tetradecen-1-ol acetate(1.88), 1-Hexadecyne (9.71), Z-(13,14-Epoxy)tetradec-11-en-1-ol acetate(2.61), Octadecanoic acid (6.01), Tridecanedial (4.78) and cis-13-Eicosenoic acid (1.17). HPLC analysis of Ensete superbum Seed Powder reported that it has mainly contains five flavonoids compounds, namely Gallic acid (5.550 min), Caffeic acid (9.233min), Rutin (10.317min), Quercetin (12.125min) and Ferulic acid (23.200min). The results of FTIR analysis confirmed the presence of Alkynes, Alkanes , Amines , aromatic amines, alkyl halides, alkenes, carboxylic acids and Aromatic Compounds. Further, proton nuclear magnetic resonance spectrum of Ensete superbum Seed Powder was recorded and the chemical shift values of the various signals are identified. The detection of these phytochemical compounds present in the medicinal plants will endow with some information on Ensete superbum Seed Powder as herbal alternative for cure vast array of diseases.

Key words: GCMS, HPLC, FTIR, HNMR and Ensete superbum

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Cite this article: Kumar, R.N., Muthukumaran, P., Suresh Kumar, K. and   Karthikeyen, R., Phytochemical Characterization of Bioactive Compound from the Ensete superbum SeedPowder, Int. J. Pure App. Biosci.6(6): 635-643 (2018). doi: