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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 1
First page : (224) Last page : (229)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7340

In Vitro Screening and Production of Plant Growth Promoting Substances by Azospirillum Isolates from Rhizoplane of Foxtail Millet
[Setaria italica (L.) Beauv]

Vijayalakshmi N. R.*, Mahadeva Swamy and Nagaraj M. Naik

Department of Agricultural Microbiology, College of Agriculture, Raichur
University of Agricultural Sciences, Raichur-584101, Karnataka India
*Corresponding Author E-mail: vijayalakshminr77@gmail.com
Received: 13.01.2019 | Revised: 17.02.2019 | Accepted: 22.02.2019  

 ABSTRACT

Isolation and identification of Azopirillum spp. with enhanced potential to promote plant growth among the natural bacterial population associated with rhizosphere soil and roots of Foxtail millet collected from forty different locations in Raichur and Koppal districts of Northern Karnataka was carried out. Further in order to select the most efficient isolates as candidates for plant growth promotion forty Azospirillum isolates were screened for nitrogen fixation and other PGPR activity. Out of forty isolates, twenty three isolates were identified as A. brasilense and remaining seventeen as A. lipoferum as well as screened for their potential nitrogen fixation, IAA and siderophore production under sterile conditions. Among 40 isolates obtained 21 were able to fix nitrogen. Nitrogen fixation ranged from 21.41 mg (MARV-18) to 2.09 mg of N per g of malate used. Twenty one isolates produced indole-3-acetic acid (IAA)ranged from 1.14 to 18.44 μg (MARV-18) per 100 ml. Six efficient Azospirillum strains produced siderophore that ranged from 47.33 to 72.67 (MARV-18) per cent. These bacterial isolates revealed potential to increase crop productivity, pot crop experiments in climatic conditions of UAS, college of agriculture was done in order to formulate recommendations for their use as inoculants.

Key words: Azospirillum, Foxtail millet, IAA, Nitrogen fixation, Siderophore.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Vijayalakshmi, N.R., Swamy, M. and Naik, N. M., In Vitro Screening and Production of Plant Growth  Promoting Substances by Azospirillum Isolates from Rhizoplane of Foxtail Millet [Setaria italica (L.) Beauv], Int. J. Pure App. Biosci.7(1): 224-229 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7340