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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 1
First page : (412) Last page : (419)
Article doi: :

Oxidative Stress in Vitamin B12 Deficient Female Subjects (30 – 50 years)

Revati Shah*, Vinayak Patel and Neeta Dave

Post Graduate Department of Home Science, Sardar Patel University,
Vallabh Vidyanagar, Anand, Gujarat, India
*Corresponding Author E-mail:
Received: 23.12.2018  |  Revised: 28.01.2019   |  Accepted: 7.02.2019  



Objective: The present study was carried out to evaluate the oxidative stress in normal and vitamin B12 deficient females (age: 30 – 50 years). Methods: 204 female subjects, aged 30 to 50 years were purposively selected from Vallabh Vidyanagar, Anand, Gujarat, India. Fasting venous blood sample was collected from all the subjects. Whole blood was estimated for Ascorbic Acid and Reduced Glutathione (GSH) levels while serum was used for the estimation of vitamin B12 and Total Antioxidant Capacity (TAC) using FRAP assay. The subjects having vitamin B12 levels >211 pg/ml were considered as normal subjects. Results: The female subjects were divided into two groups 30 – 40 years and 41 – 50 years. A significant difference (P≤0.01) was observed in vitamin B12 levels of normal and deficient female subjects belonging to both age groups. Female subjects (aged 41 – 50 years) who were having normal vitamin B12 levels were found to have significantly lower (P≤0.05) whole blood ascorbic levels (0.807 mg%) as compared to vitamin B12 deficient female subjects (1.031 mg%). Whole blood GSH levels of female subjects (30 – 40 years) who had a normal vitamin B12 levels were significantly higher (P≤0.05) compared to that of vitamin B12 deficient subjects. Serum TAC level was found to be significantly lower (P≤0.01) in vitamin B12 deficient female subjects compared to those having normal vitamin B12 levels as observed in both the groups. Conclusion: Vitamin B12 deficiency among 30 – 50 years female subjects was associated with oxidative stress as evident from a decreased level of whole blood glutathione levels and serum total antioxidant capacity.

Key words: Oxidative stress; Vitamin B12; Reduced glutathione; Total antioxidant capacity.

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Cite this article: Shah, R., Patel, V., Dave, N., Oxidative Stress in Vitamin B12 Deficient Female Subjects (30 – 50 years), Int. J. Pure App. Biosci.7(1): 412-419 (2019). doi: