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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 2
First page : (198) Last page : (208)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7417

Assessment of Phytochemicals and Antioxidant Activities of Leaf and Leaf Derived Callus Extracts of Lepianthes umbellata (L.) Raf.

Manasa Govindaraju¹, Mahendra Chikkamadaiah¹, Murali Mahadevamurthy², Vinaya³ and Sudarshana Mysore Shankar Singh¹*

¹Plant Tissue culture Lab, ²Plant Pathology Lab, Department of Studies in Botany,
Manasagangotri, University of Mysore, Mysuru-570006
³Department of Studies in Chemistry, Manasagangotri, University of Mysore, Mysuru-570006
*Corresponding Author E-mail: mssingh.uom@gmail.com
Received: 11.03.2019 | Revised: 14.04.2019 | Accepted: 19.04.2019  

 ABSTRACT

The aim of the present work is to ascertain and compare the level of phytochemical and antioxidantactivity of different solvent extracts from leaf and leaf derived callus of Lepianthes umbellata (L.) Raf. a rare medicinal plant of  Western Ghats. The phytochemical analysis indicated the presence of alkaloids, terpenoids, steroids, carbohydrates, tannins, saponins flavonoids etc., DPPH, total phenolic content, total phosphomolybdenum content, reducing power assay, antilipid peroxidation assay and nitric oxide scavenging assays were used to assess the antioxidant activity. In all the tested methods methanolic extract of leaf and leaf derived callus possessed the appreciable amount of antioxidant activity. In addition to this hemolytic activity of all the extracts was screened against normal human erythrocytes. All most all the tested extracts possessed minimum hemolytic activity. Results of the present study indicated both leaf and leaf derived callus contain natural antioxidant constituents which could be exploited for possible drug development.

Key words: Antioxidant, Callus, Lepianthes umbellata, Phytochemical.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Govindaraju, M., Chikkamadaiah, M., Mahadevamurthy, M., Vinaya and Singh, S. M.S., Assessment of Phytochemicals and Antioxidant Activities of Leaf and Leaf Derived Callus Extracts of Lepianthes umbellata (L.) Raf., Int. J. Pure App. Biosci.7(2): 198-208 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7417