INTERNATIONAL JOURNAL OF PURE & APPLIED BIOSCIENCE
ISSN : 2320-7051
International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 2
First page : (198) Last page : (208)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7417
Assessment of Phytochemicals and Antioxidant Activities of Leaf and Leaf Derived Callus Extracts of Lepianthes umbellata (L.) Raf.
Manasa Govindaraju1, Mahendra Chikkamadaiah1, Murali Mahadevamurthy2, Vinaya3 and Sudarshana Mysore Shankar Singh1*
1Plant Tissue culture Lab, 2Plant Pathology Lab, Department of Studies in Botany,
Manasagangotri, University of Mysore, Mysuru-570006
3Department of Studies in Chemistry, Manasagangotri, University of Mysore, Mysuru-570006
*Corresponding Author E-mail: firstname.lastname@example.org
Received: 11.03.2019 | Revised: 14.04.2019 | Accepted: 19.04.2019
The aim of the present work is to ascertain and compare the level of phytochemical and antioxidantactivity of different solvent extracts from leaf and leaf derived callus of Lepianthes umbellata (L.) Raf. a rare medicinal plant of Western Ghats. The phytochemical analysis indicated the presence of alkaloids, terpenoids, steroids, carbohydrates, tannins, saponins flavonoids etc., DPPH, total phenolic content, total phosphomolybdenum content, reducing power assay, antilipid peroxidation assay and nitric oxide scavenging assays were used to assess the antioxidant activity. In all the tested methods methanolic extract of leaf and leaf derived callus possessed the appreciable amount of antioxidant activity. In addition to this hemolytic activity of all the extracts was screened against normal human erythrocytes. All most all the tested extracts possessed minimum hemolytic activity. Results of the present study indicated both leaf and leaf derived callus contain natural antioxidant constituents which could be exploited for possible drug development.
Key words: Antioxidant, Callus, Lepianthes umbellata, Phytochemical.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Govindaraju, M., Chikkamadaiah, M., Mahadevamurthy, M., Vinaya and Singh, S. M.S., Assessment of Phytochemicals and Antioxidant Activities of Leaf and Leaf Derived Callus Extracts of Lepianthes umbellata (L.) Raf., Int. J. Pure App. Biosci.7(2): 198-208 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7417