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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 2
First page : (235) Last page : (241)
Article doi: :

Effect of Physical and Chemical Mutagens in Chrysanthemum (Dendranthema grandiflora Tzvelev) cv. Jaya for
Determination of Mutagenic Sensitivity

Rajasekar P.1*, Kannan M.2 and Kumar M.3

1Ph.D. scholar, Dept. of Floriculture and Landscaping, HC & RI, Coimbatore - 641 003,
2Dean (Hort.), Adhiparasakthi Horticultural College, Vellore -632 506,
3Professor (PBG), TPO to Vice Chancellor, Coimbatore - 641 003,
Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India
*Corresponding Author E-mail:
Received: 2.01.2019  |  Revised: 7.02.2019   |  Accepted: 16.02.2019  



An experiment was conducted to determine LD50 does of physical and chemical mutation in cv. Jaya. In a mutation breeding experiment, LD50 gives an indication of the response on different types of explants to mutagen, so that the right dose(s) for the main field experiment can be fixed to obtain beneficial mutants with minimal population loss. In the present investigation, herbaceous cuttings (7 - 10 cm long with 4- 5 nodes) of chrysanthemum cv. Jaya were treated with twenty one different dosages of Gamma rays and EMS ranging from 5.00 to 50.00 Gray and 0.10 to 0.50 per cent respectively. The results revealed a gradual and significant reduction based on survival rates of the treated cuttings using probit analysis as well as the growth rate in different dosage of both mutagens. All the treatments with gamma rays and EMS revealed a dose dependent negative linear relationship between dose and survival of treated cuttings. The survival percentage of variety Jaya in gamma irradiated population ranged from 85.00 per cent in 5 Gy to 20.00 per cent in 35 Gy among the gamma ray treatment. In case of EMS treatments, survival was ranged from 75.00 per cent in 0.10 per cent of EMS and 5.00 per cent in 0.45 per cent of EMS and none of the plants were survived in 0.50 percent ethyl methane sulphonate. Based on the survival of treated cuttings probit curve analysis was exhibited that, LD50 determined as 21.47 Gray for gamma radiation and 0.22 per cent for EMS.

Key words:Chrysanthemum, Ethyl Methane Sulphonate, Gamma rays, Survival, Sensitivity and Jaya

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Cite this article: Rajasekar, P., Kannan, M. and Kumar, M., Effect of Physical and Chemical Mutagens in Chrysanthemum (Dendranthema grandiflora Tzvelev) cv. Jaya for Determination of Mutagenic Sensitivity, Int. J. Pure App. Biosci.7(2): 235-241 (2019). doi: