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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 2
First page : (51) Last page : (57)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7290

In-vitro Propagation of Lavendula officinalis through Shoot Tips and Seeds

Firdous A. Mala¹* and Mushtaq A. Sofi²

Govt Degree College Handwara Kashmir, India 193221
*Corresponding Author E-mail: firdousbotany9@gmail.com
Received: 11.02.2019  |  Revised: 16.03.2019   |  Accepted: 23.03.2019  

 ABSTRACT

In the present study a successful procedure for rapid proliferation of shoots and seed germination of Lavendula officinalis Chaix was formulated. Shoot tip cultures were preferred as the pre-existing meristem easily develops into shoots while maintaining clonal fidelity. The superiority of BAP and IAA over Kn and IBA combination has been Investigated during the present study. The BAP + IAA combination showed more shoot proliferation then KN + IBA combination. Among the various: auxin cytokinin combinations, the best in terms of shoot multiplication were recorded in BAP (0.8mg/1) + IAA (0.4mg/1) after sixth days of inoculation.
In order to break the dormancy of the lavender seeds an efficient protocol was carried out under in-vitro laboratory conditions. Seeds of Lavendula officinalis subjected to scarification showed 6.25% germination in light and same percentage of seed germination was recorded in complete darkness. Seeds of Lavendula officinalis subjected to acid wash showed 12.5% germination in light. At dark treatment the seeds treated with sulphuric acid showed 6.25 % of germination.  Seeds subjected to 48 hours chilling treatment showed 25% of germination in light. The dark chilling treatment showed 18.75% of germination.  Best results (31.25%) were recorded in seeds treated with GA3 (200ppm) in ordinary light and 12.5% of seed germination was recorded in GA3 seeds kept in complete darkness.

Key words: Lavendula officinalis, Invitro propagation, Seed dormancy, Shoot tip.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Mala, F.A. and Sofi, M.A., In-vitro Propagation of Lavendula officinalis through Shoot Tips and Seeds, Int. J. Pure App. Biosci. 7(2): 51-57 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7290