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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 3
First page : (15) Last page : (24)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7464

The Hepato-Renal Protective Effect of Nelumbo nucifera Gaertn Seeds against Carbon Tetra Chloride Toxicity in Rats

Amber Ayaz Memon, Lubna Naz*, Sakina Shabbir and Zoha Khan

Department of Physiology, University of Karachi, Karachi, Pakistan
*Corresponding Author E-mail: lunasaf@yahoo.com
Received: 13.04.2019 | Revised: 20.05.2019 | Accepted: 26.05.2019  

 ABSTRACT

Nelumbo nucifera Gaertn seeds (Lotus seeds) has been used in Traditional medicine since long ago. Lotus seeds shows notable antioxidant activity attributed to presence of alkaloids, Saponins, Phenolics and anti-ageing enzyme, which is believed to repair damaged protein.
The purpose of study was to determine hepatorenal protective effect of aqueous extract of Nelumbo nucifera Gaertn (Lotus) seed on toxicity induced by Carbon tetrachloride.
Female Wistor rats 200-250g were divided into three groups (n=6). Group I remained untreated whereas Group II & III received 0.8ml/kg of CCl4 subcutaneously twice a week for 21 days. In addition, 200mg/kg of aqueous Lotus seed extract was given to Group III rats orally via gavage daily for 21 days. 0n 22ndday animals were sacrificed and blood and tissue samples were collected for             biochemical      and       histopathological           analysis.
AST levels (p>0.05), ALT levels, bilirubin levels.Urea, BUN, Creatinine levels (p< 0.05) were increased in group II (CCl4 treated rats) as compared to untreated. The N. nucifera seed extract reduced AST levels (p>0.05), ALT levels, bilirubin levels, Urea, BUN, Creatinine levels (p<0.05) in group III (CCl4 + N. nucifera Gaertn seed extract treated) rats as compared with group II showing protective effect of Lotus seeds. Elevation of body weight in group I and group  II while reduction in group III was recorded. The histology features show mild enlargement and lobular inflammation in group II and group III and no pathologic evidence was found in group I.
It is concluded that Lotus seed has profound hepatorenal protective effects.

Key words: Aqueous extract, Nelumbo nucifera, Liver enzymes, Lotus seed.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Memon, A.A., Naz, L., Shabbir, S., and Khan, Z., The Hepato-Renal Protective Effect of Nelumbo nucifera Gaertn Seeds Against Carbon Tetra Chloride Toxicity In Rats, Int. J. Pure App. Biosci.7(3): 15-24 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7464