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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 3
First page : (325) Last page : (328)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7344

Applicability of Polymerase Chain Reaction for the Detection of Meat Species in Dried Animal Tissues (pet chews)

K. Sudheer*, R. Narendra Babu, V. Appa Rao, S. Ramesh and T.M.A. Senthil Kumar

Department of Livestock Products Technology (Meat Science)
Madras Veterinary College, Chennai - 600007
Tamil Nadu Veterinary and Animal Sciences University
*Corresponding Author E-mail: drsudhirkorukonda@gmail.com
Received: 9.03.2019 | Revised: 15.04.2019 | Accepted: 23.04.2019  

 ABSTRACT

Polymerase chain reaction technique was employed to identify the meat species of dried animals tissue like trachea, oesophagus, ligamentum nuchae, penis, ears obtained from meat processing plants which are used as pet chews for feeding of pet animals. The simultaneous detection of both the species cattle and buffalo at a time was achieved by conducting duplex polymerase chain reaction. Using mitochondrial sequences, species specific primers of cyt b gene was used for cattle and buffalo primer at 274 bp and 425 bp respectively. All the samples screened were tested positive for buffalo species which could be due to the ban of cow slaughter and it’s processing in India.

Key words: Pet chews, Cyt b gene, Cattle, Buffalo.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Sudheer, K., Narendra Babu, R., Appa Rao, V., Ramesh, S. and Senthil Kumar, T. M. A., Applicability of Polymerase Chain Reaction for the Detection of Meat Species in Dried Animal Tissues (pet chews), Int. J. Pure App. Biosci.7(3): 325-328 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7344