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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 3
First page : (335) Last page : (340)
Article doi: :

Cultural Study of Fire-fang Isolates of Mushroom Compost

Prerna Dobhal and Sarvesh Kumar Mishra*

Department of Plant Pathology, College of Agriculture, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand
*Corresponding Author E-mail:
Received: 8.05.2019 | Revised: 3.06.2019 | Accepted: 7.06.2019  



Six individual colonies of fire-fang assigned as FI1, FI2, FI3, FI4, FI5 and FI6 were isolated from the four samples of hot compost having 55oC- 60oC temperature. Each one of 1st three samples was drawn at the end of turning 1st (FI1), turning 2nd (FI2), turning 3rd (FI3 and FI4) of phase I composting. Fourth compost sample represents phase II composting (FI5 ­and FI6). All isolates were found Gram (+) and appeared in white, dry and rough to smooth surface on actinomycetes isolation agar (AIA) medium but varied in shape from circular (FI1, FI3 and FI6) to irregular (FI2, FI4 and FI5) and diffusible pigmentation of pale yellow (FI1), yellow (FI5 ­and FI6), dark brown (FI2) and brown (FI3 and FI4). The effect of temperature and media on the growth of above isolates was further evaluated with the results that temperature 35oC±1 did not support any growth of above isolates. Temperature 55oC±1has obviously increased average diametric growth of all isolates of fire-fang by 122.61% against temperature 45oC±1 at 10th DAI. Starch ammonium agar (SAA) supported 9.83% more average diametric growth of all isolates of fire-fang at 10 DAI over the actinomycete isolation agar (AIA) medium. Though, the interactions FI2*55°C, FI3*55°C, FI5*55°C and FI2*KM, FI3*AIA were appeared best to have highest average growth of thermophilic isolates.

Key words: Mushroom compost, Agaricus bisporus, Fire-fang, media, Temperature, Thermophilic actinomycetes.

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Cite this article: Dobhal, P. and Mishra, S. K., Cultural Study of Fire-fang Isolates of Mushroom Compost, Int. J. Pure App. Biosci.7(3): 335-340 (2019). doi: