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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 3
First page : (341) Last page : (345)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7559

Phylogenetic Analysis of 16S Ribosomal DNA Sequences of Uncultured Bacterial Communities from the Creatceous Fossil
Sediments of Ariyalur Basin

Edward A.*

Assistant Professor, Department of Biotechnology, St.Joseph’s College, Tiruchirapalli, Tamil Nadu, India
*Corresponding Author E-mail: cisgene.edward@gmail.com
Received: 5.05.2019  |  Revised: 3.06.2019   |  Accepted: 8.06.2019  

 ABSTRACT

Metagenomics is a process which involves the bypassing of microbial cultures in the laboratory conditions and enables to study the microbial communities that are difficult to culture. In the present investigation, uncultured microbial DNA was isolated from the fossil sediments and characterized through 16S rRNA gene sequencing. Two different 16S rRNA gene amplicons were cloned in the vector respectively and subjected to 16S rRNA sequencing. Bioinformatics analysis of two 16S rRNA gene sequences has shown the closer resemblance to Brevibacterium species and Pseudomonas species respectively. The microbial data suggest the sea invasion theory of Ariyalur.

Key words: Fossils, Ariyalur, Pseudomonas species, Brevibacterium species, 16S ribosomal RNA.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Edward, A., Phylogenetic Analysis of 16S Ribosomal DNA Sequences of Uncultured Bacterial Communities from the Creatceous Fossil Sediments of Ariyalur Basin, Int. J. Pure App. Biosci. 7(3): 341-345 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7559