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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 4
First page : (190) Last page : (202)
Article doi: :

Nutraceutical Evaluation of Horse Gram (Macrotyloma uniflorum)  Cultivated in High Altitudes of Uttarakhand Himalaya, India

Netrapal Sharma1*, Satpal Singh Bisht1, Sanjay Gupta2, Mahendra Rana3 and Ajay Kumar1
1*Department of Zoology, 3Department of Pharmaceutical Sciences,
Kumaun University, Nainital – 263002, Uttarakhand, India
2Himalayan School of Biosciences, Swami Ram Nagar, Beside Jolly Grant Airport,
Jolly Grant, Doiwala, Dehradun, Uttarakhand, 248016, India
*Corresponding Author E-mail:
Received: 3.07.2019 | Revised: 8.08.2019 | Accepted: 17.08.2019  


Complete exploration of nutraceutical properties of Macrotyloma uniflorum by liquid chromatography mass spectrometry (LC-MS) and proximate analysis was performed. Seeds of M. uniflorum were collected from local farmers from the higher altitude (2178m, N-29°27.034’; E-079°46.210’). Three types of extracts were prepared with the help of soxhlet extractor and dried in vacuum dryer. The above extracts were examined by LC-MS (TOF/Q-TOF Mass Spectrometer) for the presence of alkaloids, glycosides, saponins, phenolic compounds, tannins followed by Macronutrients, micronutrients and proximate analysis followed by LC-MS analysis in Hydrolic (66), Ethanolic (66) and Methanolic extract (119). The maximum number of compounds was found in methanolic extract, compounds common in all solvents were (32), common in methanolic and ethanolic were (17) and common in methanolic and hydrolic were (11) respectively. The unique compounds found in methanolic; ethanolic and hydrolic were 58, 17, 23 respectively. Sizable numbers of metabolic compounds were found in all extracts those are considered as very useful compounds in various metabolic regulations specifically in anti-lithiasis, anti-obesity, anti-diabetes, Alzheimers disease and many other neurological disorders. Present investigation is very significant in terms of exploring the nutraceutical properties of M. uniflorum and the outcome of this study would be of wide interest to farmers and researchers working on nutraceuticals including commercial explorations.

Keywords: LC-MS, Proximate analysis, Anti-lithiasis, Anti-obesity, Anti-diabetes, Macrotyloma uniflorum.

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Cite this article: Sharma, N., Bisht, S. S., Gupta, S., Rana, M., & Kumar, A., (2019).  Nutraceutical Evaluation of Horse Gram (Macrotyloma uniflorum) Cultivated in High Altitudes of Uttarakhand Himalaya, India, Ind. J. Pure App. Biosci. 7(4), 190-202. doi: