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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 4
First page : (319) Last page : (328)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7737

Isolation and Characterization of Endophytic Bacteria from Nodule, Root and Seeds of Greengram (Vigna radiata L.)

M. Bhagya¹, K. Nagaraju², B. J. Praveen Biradar^3*, G. P. Santhosh⁴ and R.C. Gundappagol^5
1Assistant Professor, Department of Agricultural Microbiology, Agricultural College, Kalaburagi
²Assistant Professor, Department of Agricultural Microbiology, University of Agriculture sciences, Bengaluru
³Assistant Professor, Department of Agricultural Microbiology, Agricultural College, Vijayapur
⁴Assistant Professor, Department of Agricultural Microbiology, University of Agriculture sciences, Dharwad
⁵Professor of Department of Agricultural Microbiology, College of Agriculture, Kalaburagi
*Corresponding Author E-mail: pavitheking21@gmail.com
Received: 26.06.2019  |  Revised: 29.07.2019   |  Accepted: 10.08.2019  

 ABSTRACT

Plants are host to one or more endophytic microorganisms. However the endophyte plant interaction is one of the least studied biochemical system in nature. The present experiment was conducted to isolate endophytic bacteria from surface sterilized nodules, roots and seeds of Greengram. Total 10 endophytic bacteria were obtained from nodules, roots and seeds of Greengram. Highest population of endophytic bacteria were observed in nodules (7.5 ×105 CFU/g) followed by root (5.6×105 CFU/g) and seeds (3.2×105 CFU/g). Based on microscopic observation it was found that all the 10 isolates were rod shaped, when studied for gram reaction out of 10 isolates 8 isolates showed negative reaction, out of ten 6 isolates were motile and remaining 4 isolates were non motile and with regards to endospore formation test 4 isolates were observed as endospore forming bacteria and remaining 6 isolates were non endospore formers. With respect to biochemical characterization all isolates showed positive results for catalase test, citrate utilization, phosphate solubilization and casein production tests and all isolates showed negative result for Indole production and Voges-Proskauer test.

Keywords: Endophytic bacteria, Morphological and Biochemical characterization.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Bhagya, M., Nagaraju, K., Praveen Biradar, B. J., Santhosh, G.P., & Gundappagol, R.C. (2019). Isolation and Characterization of Endophytic Bacteria from Nodule, Root and Seeds of Greengram (Vigna radiata L.), Ind. J. Pure App. Biosci. 7(4), 319-328.   doi: http://dx.doi.org/10.18782/2320-7051.7737