INDIAN JOURNAL OF PURE & APPLIED BIOSCIENCES

ISSN (E) : 2582 – 2845

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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 5
First page : (259) Last page : (264)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7838

Simple Sequence Repeat (SSR) Marker Based Genetic Diversity Analysis in White Yam (Dioscorea rotundata Poir.)

Parvathy Harikumar* and Sheela M. N.
Division of Crop Improvement, ICAR-Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram, Kerala, India, 695017
*Corresponding Author E-mail: coolfrnzparu@gmail.com
Received: 13.09.2019  |  Revised: 17.10.2019   |  Accepted: 24.10.2019  

ABSTRACT

Diversity analysis of the genotypes helps to develop accurate breeding program along with the identification of elite genotypes from the cultivars. ICAR-Central Tuber Crops Research Institute, Kerala holds a field gene bank of 1100 Dioscorea accessions including 158 white yam genotypes. Present study focused on identifying genetic variability in 30 white yam genotypes (Dwarf, Semi dwarf and Tall) using 14 SSR primers. From 14 SSR primers, only 10 primers were selected based on their polymorphism and repeatability. Among the 10 SSR primers studied in white yam, YM15 recorded the maximum number of polymorphic alleles of five followed by    Dab2D06 and YM26 with four alleles. The percentage of polymorphism ranged from 50 to 100. The observed heterozygosity values ranged from 0.4339 (Dab2C05) to 0.775 (YM 15). The polymorphism information content ranged from 0.3398 (Dab2C05) to 0.7388 (Dab2D06). YM15 also recorded high PIC value of 0.7377. Dendrogram based on SSR marker showed that white yam genotypes were grouped in to three clusters. It also shows the high divergence of the genotype V20 (DR17) and the clustering pattern indicates the grouping of the dwarf genotypes together along with a semi dwarf variety.

Keywords: Yams, Genetic variability, SSR markers, Dendrogram, Dwarf genotype and Heterozygosity.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Harikumar, P. & Sheela, M. N. (2019). Simple Sequence Repeat (SSR) Marker Based Genetic Diversity Analysis in White Yam (Dioscorea rotundata Poir.), Ind. J. Pure App. Biosci.7(5), 259-264. doi: http://dx.doi.org/10.18782/2320-7051.7838




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