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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 5
First page : (360) Last page : (363)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7842

Phosphate Solubilisation Potential of Screened Nitrogen Fixing Rhizobium leguminosarum Strains Isolated from Nodules of Pea Plant

Srishty¹, Anshu Sibbal Chatli^2* and Parminder Kaur^1
1Department of Biotechnology, ^2*Department of Microbiology,
Guru Nanak Girls College, Model Town, Ludhiana (Punjab)
*Corresponding Author E-mail: manansh@hotmail.com
Received: 4.09.2019  |  Revised: 10.10.2019   |  Accepted: 16.10.2019  

 ABSTRACT

The present study was undertaken to isolate Rhizobium leguminosarum strains from the nodules of Pea (Pisum sativum (L)) var AP-3 grown in the soil of Hoshiarpur. These isolated nitrogen fixing strains were also tested for their tendency to solubilize Tricalcium Phosphate (TCP) on Pikovskaya (PVK) agar, Modified Pikovskaya (MPVK) agar and National Botanical Research Institute (NBRIP agar). The halo size of R. leguminosarum SS-8 was reported to be biggest (13 mm) on NBRIP agar and the smallest (4 mm) in PVK agar. These strains were further inoculated in PVK and NBRIP broths, respectively to see their potentials to solubilize TCP. The strain R. leguminosarum SS-8 was observed to show maximum TCP solubilisation (261 µg/ml in NBRIP and 211 µg/ml in PVK broths) with maximum decrease in pH (4.14 in NBRIP and 4.20 in PVK broths).

Keywords: Rhizobium, Nitrogen fixation, PVK, MPVK, NBRIP.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Srishty, Chatli, A.S., & Kaur, P. (2019). Phosphate Solubilisation Potential of Screened Nitrogen Fixing Rhizobium leguminosarum Strains Isolated from Nodules of Pea Plant, Ind. J. Pure App. Biosci. 7(5), 360-363. doi: http://dx.doi.org/10.18782/2320-7051.7842