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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 5
First page : (499) Last page : (503)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7839

Up-regulation of Serum microRNA-21 in Patients with Pulmonary Tuberculosis (PTB) as Compare to Healthy Individuals

Aditya Upadhyay¹, Anmol Kulshrestha¹, R.R.K. Niraj², Deepika Gupta⁴, Arvind Jat⁴, Mrituanjai Singh¹, Archana Tiwari¹, Shubhra Jain³ and Sandeep K. Shrivastava^4*
1School of Biotechnology – Rajiv Gandhi Proudyogiki Vishwavidyalaya, Airport Road, Bhopal, 462033, India
²Dr. B. Lal Institute of Biotechnology, Jaipur, India
³Department of Respiratory Medicine, Sawai Man Singh (SMS) Medical College, Jaipur, 302004, India
⁴Centre for Innovation Research and Development (CIRD), Dr. B. Lal Institute of Biotechnology,
Dr. B. Lal Clinical Laboratories Pvt. Ltd., Jaipur, 302017, India
*Corresponding Author E-mail: ssk.cird1@gmail.com
Received: 10.09.2019  |  Revised: 17.10.2019   |  Accepted: 24.10.2019  

 ABSTRACT

Tuberculosis is a lethal disease worldwide due to absences of early diagnostic processes. microRNAs have been denoted their functional and diagnostic potential in various diseases like  cancer and tuberculosis. In the present study, we performed the miRNA isolation from the clinical confirm pulmonary tuberculosis (PTB) and healthy control cases. We have observed the up-regulation of serum microRNA-21 in serum sample of pulmonary tuberculosis (PTB) patients. Our results based on qRT-PCR confirmation and receiver operational curve (ROC) analysis indicate that the serum microRNA-21 can be explored as potential non invasive biomarker for early detection of pulmonary tuberculosis infection in human.

Keywords: Pulmonary Tuberculosis. microRNA-21, Non-Invasive Biomarker, Up-regulation, ROC.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Upadhyay, A., Kulshrestha, A., Niraj, R.R.K., Gupta, D., Jat, A., Singh, M., Tiwari, A., Jain, S., & Shrivastava, S.K. (2019). Up-regulation of Serum microRNA-21 in Patients with Pulmonary Tuberculosis (PTB) as Compare to Healthy Individuals, Ind. J. Pure App. Biosci.7(5), 499-503. doi: http://dx.doi.org/10.18782/2320-7051.7839