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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 6
First page : (367) Last page : (372)
Article doi: : http://dx.doi.org/10.18782/2582-2845.7928

In vitro Isolation and Optimization of Favourable Culture Conditions for the Mycelial Growth of Amanita ceciliae – A Mycorrhizal Mushroom

Amit Kumar Sehgal^1* and Anand Sagar^2
1Department of Botany, Govt. College Dhaliara, District Kangra (H.P.) 177103 India
²Department of Biosciences, Himachal Pradesh University, Summer Hill Shimla (H.P.) 171005 India
*Corresponding Author E-mail: draksehgal@gmail.com
Received: 10.11.2019  |  Revised: 15.12.2019   |  Accepted: 23.12.2019  

 ABSTRACT

In the present study, different solid media were evaluated for the best mycelial growth of Amanita ceciliae, out of ten solid media tested, A. ceciliae showed maximum mycelial growth on Potato Dextrose Agar Medium. Various factors such as temperature, pH, light and darkness were also investigated. The best mycelial growth was observed at 20°C. To determine the optimum pH, the mycelium was grown in best suited solid media at optimum temperature and at different pH levels. Maximum mycelial growth was achieved at pH 5.0 whereas the mycelium was found to grow better under the dark conditions in comparison to light.

Key words: Amanita ceciliae, Vitro isolation, Cultural conditions, Mycelial growth.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Sehgal, A.K. & Sagar, A. (2019). In vitro Isolation and Optimization of Favourable Culture Conditions for the Mycelial Growth of Amanita ceciliae – A Mycorrhizal Mushroom, Ind. J. Pure App. Biosci. 7(6), 367-372. doi: http://dx.doi.org/10.18782/2582-2845.7928