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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 6
First page : (386) Last page : (401)
Article doi: : http://dx.doi.org/10.18782/2582-2845.7768

Isolation and Identification of Arbuscular Mycorrhizal (AM) Fungi from The Root Zone Soils of 11 Different Fruit Crops Grown in the Central Campus, MPKV, Rahuri

Someshwar  Raut^1*, Chintamani Deokar¹, Annasaheb Navale² and Jayshri Dahatonde^3
1Ph.D scholar, ²Associate Professor
^1,2Department of Plant Pathology and Agril. Microbilogy, Post Graduate Institute, MPKV, Rahuri
¹Professor of Plant Pathology, Plant Pathology and Agril. Microbiology Section, College of Agriculture, Dhule
³Assistant Professor, Department of Agril. Entomology, DVVPF’s College of Agriculture, Ahmednagar
*Corresponding Author E-mail: someshwar844@gmail.com
Received: 23.09.2019  |  Revised: 29.10.2019   |  Accepted: 7.11.2019  

 ABSTRACT

The aim of the present study was to isolate AM fungi from the root zone soils of 11 different fruit crops grown in the central campus, MPKV, Rahuri.
The chemical properties of the root zone soils of the collected sites which included, soil pH, organic carbon, available nitrogen, available phosphorus and micro-nutrients (Fe, Mn, Zn and Cu). As the soil pH is concerned, majority of soils are slightly alkaline (7.1-8.0). Strongly alkaline soil pH (8.58) was recorded in (Block- E, Survey no. 84) and neutral soil pH (7.40) in (Block- E, Survey No. 92).
Soil analysis for organic carbon content showed low to moderate status. The highest organic carbon status was recorded in Block: HF Survey no. 57 (0.66 %) and lowest in Block: E Survey no.78 and survey no. 94 and Block : B Survey no.24 (0.20 %).
 The available nitrogen content in the present study ranged from 110 and 225 kg ha-1 i.e. very low to low nitrogen content as per six tier rating. The least and highest values of available phosphorus ranged between 10 and 28 kg ha-1. Soil samples showed low to moderate ratings as per six tier rating.
In the present study, micronutrients viz. Fe, Mn, Zn and Cu were determined from 77 root zone soil samples collected. 83.12 per cent of soil samples showed deficit in Fe content, 100 per cent soil samples were deficit in Mn content, 81.82 per cent  of soil samples showed deficit in Zn content, where as 3.99 per cent of soil samples showed deficit in Cu content.
A total of 11 AM fungal species were isolated from 77 different root zone soil samples belonging to five different genera viz., Glomus, Aculospora, Gigaspora, Sceutellospora and Rhizophagus. Glomus was the dominant genus followed by Aculospora, Sceutellospora, Rhizophagus and Gigaspora. Glomus mosseae was the most dominant species was recorded. A number of spore morphotypes were detected at each site, according to shape, colour and size. All the spores belonged to Glomerales and Diversisporales orders of AM fungi.  A total of 4,243 spores of AMF were wet sieved from the soil samples collected.

Keywords: AM fungi, Chemical properties, Micronutrients and Root zone soil.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Raut, S., Deokar, C., Navale, A., & Dahatonde, J. (2019). Isolation and Identification of Arbuscular Mycorrhizal (AM) Fungi from the Root Zone Soils of 11 Different Fruit Crops Grown in the Central Campus, MPKV, Rahuri, Ind. J. Pure App. Biosci. 7(6), 386-401. doi: http://dx.doi.org/10.18782/2582-2845.7768