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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 6
First page : (47) Last page : (52)
Article doi: : http://dx.doi.org/10.18782/2582-2845.7704

Lipolytic Activity of Lactic Acid Bacteria Isolated from Different Dairy Samples

Manvar A. V.¹ and Sonwane P. A.^2*
1Associate Professor, ²Reserch Scholar
Department of Microbiology, DSM college of Arts, Commerce and Science Parbhani Maharashtra, India
*Corresponding Author E-mail: priyatam3@gmail.com
Received: 18.08.2019  |  Revised: 25.09.2019   |  Accepted: 1.10.2019  

 ABSTRACT

Lactic acid bacteria isolated from various dairy sampled were screened for lipolytic activity. Among 32 dairy samples, 24 strains of Lactic acid bacteria were obtained on MRS agar, out of them seven strains were screened as lipase producer on tributyrin agar media. These seven isolates were tested morphologically and Biochemically and identified as Lactobacillus sp. To screen best possible bacteria having lipolytic activity, qualitative screening method is performed using disc diffusion method on olive oil agar containing methyl red. Total three strains of Lactobacilli designated as L13, L17 and L18 were obtained with maximum lipolytic activity. Three strains L13, L17 and L18 were subjected for lipase production using submerged fermentation process and lipase activity was observed. Strain L13 and L18 showed maximum lipase activity 5.47 U/ml and 5.19 U/ml, while L17 showed minimum lipase activity 3.02 U/ml.

Keywords: LAB (Lactic acid Bacteria), MRS, Tributyrin, Lipolytic activity etc.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Manvar, A.V. & Sonwane, P.A. (2019). Lipolytic Activity of Lactic acid Bacteria Isolated from Different Dairy Samples, Ind. J. Pure App. Biosci. 7(6), 47-52. doi: http://dx.doi.org/10.18782/2582-2845.7704