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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 2
First page : (21) Last page : (32)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8009

A Comparative Study of Genetic Diversity Analysis through Molecular Markers and D² Statistic in Germplasm Accessions of Green Gram [Vigna radiata (L.)]

Kanavi M.S.P.^1* , Somu G.², Nagesha N.³, Krishnaprasad B.T.⁴ and N. Marappa^5
1Dept.of Genetics and Plant Breeding, College of Agriculture, Hassan,
²Assistant Breeder, AICRP on Sorghum, Chamarajanagara,
³Dept. of Plant Biotechnology, College of Agriculture, G.K.V.K,
⁴Dept. of Agricultural Biotechnology, College of Agriculture, Hassan,
⁵Dept. of Genetics and Plant Breeding, College of Agriculture, G.K.V.K,
University of Agricultural Sciences, Bangalore, Karnataka
*Corresponding Author E-mail: kanavi.uasb@gmail.com
Received: 20.02.2020  |  Revised: 26.03.2020   |  Accepted: 3.04.2020 

 ABSTRACT

Genetic diversity analysis was carried out among fifty-six germplasm accessions of green gram through simple sequence repeats (SSR) markers and Mahalanobis D² statistic for quantitative traits.  The D² statistic performed on 20 quantitative traits grouped all 56 genotypes into eight divergent clusters. Cluster V (8559.766) had maximum intra-cluster distance while inter-cluster distance was highest between clusters III and VI(329997.938)  Cluster means indicated that germplasm lines falling in cluster III and V possessed higher values for quantitative traits such as  plant height, plant diameter, number of pods per cluster, number of pods per plant, number of seeds per pod, pod yield per plant, pod length, threshing %, leaf area, specific leaf weight, leaf area ratio, seed yield per plant, seed yield per plot and biological yield. Molecular characterization was done with fifteen standardized SSR primers. All primers showed scorable polymorphism and the information was used to calculate Jackard’s similarity matrix using NTSYS-pc version 2. The UPGMA dendrogram based on SSR results divided the 56 green gram genotypes into eight main clusters under which the cluster I was highly diverse compared to all other clusters and consisted of 15 genotypes, followed by cluster II with 9 genotypes.

Keywords: Green gram germplasm, Genetic diversity, Dendrogram, SSR markers, Mahalanobis D² statistic

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kanavi, M.S.P., Somu, G., Nagesha, N., Krishnaprasad, B.T., & N. Marappa, N. (2020). A Comparative Study of Genetic Diversity Analysis through Molecular Markers and D2 Statistic in Germplasm Accessions of Green Gram [Vigna radiata (L.)], Ind. J. Pure App. Biosci. 8(2), 21-32. doi: http://dx.doi.org/10.18782/2582-2845.8009