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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 5
First page : (313) Last page : (319)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8309

Molecular Diversity Analysis in Dhaincha (Sesbania cannabina L.) Using RAPD Primers

Sangani J. L.* , Madhu Bala, Vadodariya G.D. and Patel R. K.
Department of Genetics and Plant Breeding, N. M. College of Agriculture,
Navsari Agricultural University, Navsari, Gujarat, 394 650
*Corresponding Author E-mail: jsangani51@gmail.com
Received: 1.08.2020 | Revised: 4.09.2020 | Accepted: 12.09.2020 

 ABSTRACT

Dhaincha belonging to family fabaceae is a widely grown green manure crop. In present study random amplified polymorphic DNA (RAPD) markers were used (A total of eight primers generated reproducible RAPD patterns from twenty screened primers). PCR amplification using these RAPD primers yielded seventy scorable bands, of which sixty seven were polymorphic, with an average of 8.7 polymorphic fragments per primer. Number of amplified fragments ranged from 4 (OPA-11) to 16 (OPA-13). Percentage polymorphism ranged from 75% (OPA-11) to 100 % (OPA-1, OPA-2, OPA-4, OPA-8 and OPA-11), with an average of 95.71%. The PIC value ranges from 0.203 (OPA-11) to 0.762 (OPA-3). The Jaccard’s similarity indices based on RAPD profiles were subjected to UPGMA cluster analysis and genotypes grouped in two major clusters and seven sub clusters. Clustering pattern was correlated with geographical distribution of the genotypes. Twenty nine out of thirty genotypes grouped to cluster II. This indicated the presence of narrow genetic base of the genotypes used in the study.

Keywords: Dhaincha, RAPD, Polymorphic.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Sangani, J.L., Bala, M., Vadodariya, G.D., & Patel, R.K. (2020). Molecular Diversity Analysis in Dhaincha (Sesbania cannabina L.) Using RAPD Primers, Ind. J. Pure App. Biosci. 8(5), 313-319. doi: http://dx.doi.org/10.18782/2582-2845.8309