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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2021, Volume : 9, Issue : 2
First page : (167) Last page : (172)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8634

Biochemical Marker for Establishing Distinctiveness of Ethiopian Mustard (Brassica crainata A. Braun) Varieties as Supplementary Descriptors for Plant Variety Protection

Nitin Kumar¹, Chander Mohan^2* and Sunil Kumar^3
1Department of Seed Science and Technology,
G. B. Pant University of Agriculture and Technology, Pantnagar 263 145, India
²Assistant Commissioner (Seeds), DAC & FW,
Ministry of Agriculture Cooperation and Farmers Welfare, Government of India, New Delhi 110001
³Department of Vegetable Science, CoH, Banda University of Agriculture and Technology, Banda 210001 India
*Corresponding Author E-mail: chhander@yahoo.com
Received: 16.02.2021 | Revised: 20.03.2021 | Accepted: 27.03.2021 

 ABSTRACT

India has enacted a sui generislegislation as protection of Plant Varieties and Farmer’s Rights Act 2001 (PPV&FR) for the protection of plant varieties by registration. Under PPV&FR Act DUS (Distinctiveness, Uniformity and Stability) testing procedure will be perform on the basis of morphological descriptors. Utilization of biochemical marker in DUS testing for establishing distinctiveness as a supplement to morphological descriptors has been approached in this study. Ten released cultivar and advance line of Ethiopian mustard (Brassica carinata A. Braun) were studied for morphological descriptors and total soluble proteins as biochemical marker tounveil distinctive features. SDS-PAGE for total soluble protein analysis revealed moderate degree of polymorphism. UPGMA analysis based on SDS-PAGE banding pattern data of different proteins could discriminate only three varieties Jayanti (released cultivar), PBC-2005-1 and PBC-2006-4 (advance lines). So, it can be concluded that in situations where the morpho-physiological DUS descriptors are not able to establish distinctiveness of a variety then biochemical markers may be used as additional or supplementary descriptors for unveil distinctiveness of Ethiopian mustard.

Keywords: Ethiopian Mustard, DUS, biochemical marker, SDS-Page.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kumar, N., Mohan, C., & Kumar, S. (2021). Biochemical Marker for Establishing Distinctiveness of Ethiopian Mustard (Brassica crainata A. Braun) Varieties as Supplementary Descriptors for Plant Variety Protection, Ind. J. Pure App. Biosci. 9(2), 167-172. doi: http://dx.doi.org/10.18782/2582-2845.8634