ISSN (E) : 2582 – 2845

  • No. 772, Basant Vihar, Kota

    Rajasthan-324009 India

  • Call Us On

    +91 9784677044


Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2021, Volume : 9, Issue : 3
First page : (39) Last page : (47)
Article doi: :

Next Generation Sequencing Approaches Deciphering Hidden Microbial Treasure in Soil

Ruchi Srivastava1,2, Alok Kumar Srivastava2, Pramod W. Ramteke11,3* , Vijai Kumar Gupta4 and Csaba Vágvölgyi5
1Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj, 211007, India
2ICAR-National Bureau of Agriculturally Important Microorganisms, Mau, 275103, India
3Department of Molecular Biology & Genetic Engineering,
Rashtrasant Tukadoji Maharaj Napgur University, Nagpur, 440033, India
4Centre for Safe and Improved Food & Bio-refining and Advanced Biomaterials Research Centre
Scotland’s Rural College (SRUC), Edinburgh EH9 3JG, Scotland UK
5Department of Microbiology, Faculty of Science and Informatics,
University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary
*Corresponding Author E-mail:
Received: 3.05.2021 | Revised: 7.06.2021 | Accepted: 12.06.2021 


Soil is one of the most important and complex biological habitats on earth. As we know the microbes are important key players in every ecosystem, and biological and ecological processes. Thus, it is necessary to understand this microbial treasure to have information about their role in such processes. Initial culture dependent methods helped a lot but are insufficient to indentify all the microbial species present in the soil. It has been estimated that only ~1% of bacterial species are cultivable on culture medium and rest are still hidden in through such methods. On the other hands, soil metagenomics is a modern concept that allows us to recognize these hidden species without biasness of growing bacteria on to petri plates. In last two decades rapid improvements in modern techniques itself enhanced the human capabilities in not only identifying but also have an understanding of functional aspects of these microbes in soil. Present review describes the available culture dependent methods and emergence and improvement in modern sequencing approaches helping to explore soil microbial diversity of more detail.

Keywords: Diversity, Metagenomics, Sequencing.

Full Text : PDF; Journal doi :

Cite this article: Srivastava, R., Srivastava, A. K., Ramteke, P. W., Gupta, V. K., & Vágvölgyi, C. (2021). Next Generation Sequencing Approaches Deciphering Hidden Microbial Treasure in Soil, Ind. J. Pure App. Biosci. 9(3), 39-47. doi: