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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2025 , Volume 13, Issue 4
Page No. : 1-8
Article doi: : http://dx.doi.org/10.18782/2582-2845.9166
An Assessment of Mungbean [Vigna radiata (L.) Wilczek] Genotypes Using Seed Storage Protein Profiles
1Department of Botany, University of Rajasthan, Jaipur
2Department of Plant Breeding and Genetics, SKNCOA (SKNAU)- Jobner, Jaipur, Rajasthan
*Corresponding Author E-mail: krkrishna4@rediff.com
Received: 23.05.2025 | Revised: 28.07.2025 | Accepted: 12.08.2025
ABSTRACT
Twenty genotypes and 8 market samples of mungbean were evaluated on the basis of their seed storage protein (SSP) subunits profile determined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE). Subunit bands in the range of 14kD to 80kD of molecular weight (MW) were recognized in their protein samples and appeared to show four distinct regions. Region I subunit bands were invariably monomorphic. Eight bands belonging to regions II (42kD-56kD), III (25kD-35kD) and IV (14-22 kD) were polymorphic. From binary data generated from polymorphic bands over the genotypes and market samples, Jaccard’s similarity coefficients were computed using NTSYS-pc software and utilized for the dendrogram construction. The dendrogram segregated the 20 genotypes and 8 market samples into 4 clusters. Cluster I included all the genotypes (except RMG 1004) and two market samples. The remaining 6 market samples formed clusters II and III. Genotype RMG 1004 represented cluster IV with maximum divergence.
Keywords: Mungbean, clustering, dendrogram, electrophoresis, seed storage proteins (SSP).
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2582-2845.9166
Cite this article:
Mahati, K., & Krishna, K.R. (2025). An Assessment of Mungbean [Vigna radiata (L.) Wilczek ] Genotypes Using Seed Storage Protein Profiles, Ind. J. Pure App. Biosci. 13(4), 1-8. doi: http://dx.doi.org/10.18782/2582-2845.9166
