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In order to explore the possibility of pushing up the growth and yield attributes of wheat, therefore the present investigation entitled ‘‘Growth and Yield response of wheat (Triticum aestivum L.) in relation to use of varities and bio-fertilizer” has been conducted at the AKS University sherganj satna during rabi season 2020-2021. Under the present investigation, four wheat varieties were evaluated are V₁- GW 322, V₂- JW 17, V₃- JW 3020 and V₄- JW 3288, while three levels of biofertilizers and treatments were B₁- Azotobacter (10 ml/kg seed) + 500 ml/acre foliar application, B₂- PSB (10 ml/kg seed) + 500 ml/acre foliar application and B₃- ZSB (10 ml/kg seed) + 500 ml/acre foliar application. Highest plant height (83.66 cm), number of tillers per meter row length (66.47) at 90 DAS while, number of spikes per plant (21.00), spike length (13.53 cm), number of grains per spike (29.40), test weight (41.36 g), grain yield (38.95 q/ha), stover yield (68.21 q/ha) were recorded under the wheat variety GW- 322 sown with biofertilizer of Azotobacter @ 10 ml/kg seed inoculation + 500 ml/acre foliar application.

 Keywords: Varieties, Biofertilizers, Tillers, Spike, Test weight, Stover yield.