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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2017 , Volume 5, Issue 6
Page No. : 762-764
Article doi: : http://dx.doi.org/10.18782/2320-7051.6116
Detection of Phytoplasma Diseases in Sesamum through PCR
Karra Ravindar*
Department of Biotechnology, Kakatiya University, Warangal, Telangana
*Corresponding Author E-mail: rawinder.k@gmail.com
Received: 21.11.2017 | Revised: 18.12.2017 | Accepted: 24.12.2017
ABSTRACT
Phyllody is a destructive disease of sesamum, Phyllody, Floral virescence and bushy appearance are the most common symptoms produced by phytoplama. Phytoplamal infection from sesamum was detected by PCR technique. In the present investigation amplified product of expected size is 1.2 kb from phytoplasma infected samples by PCR by using primer pair with R16F2/R16R2 for specific for the 16S rDNA sequences of phytoplasma. The PCR based techniques are very much useful for eradication and produce pathogen free planting material of sesamum.
Key words: rDNA, PCR based techniques, Sesamum, Phyllody
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.6116
Cite this article:
Ravindar, K., Detection of Phytoplasma Diseases in Sesamum through PCR, Int. J. Pure App. Biosci. 5(6): 762-764 (2017). doi: http://dx.doi.org/10.18782/2320-7051.6116
