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To study the effect of biofertilizers and varieties on growth and yield of rice, an experiment was conducted at Instructional Farm of Department of Agronomy, Faculty of Agriculture, AKS University, Sherganj, Satna (M.P.) during kharif season of 2020-21. The experiment consisted of randomize block design having Factorial arrangement with three replications. In this experiment, 12 treatment combinations including four levels of biofertilizers and treatments were B₁- PSB @ 250 g/ ha, B₂- ZSB @ 250 g/ ha, B₃- KSB @ 250 g/ ha and B₄- Azospirillum 250 ml/ha, while three rice varieties were tested are V₁- IR- 64, V₂- MTU- 1010 and V₃- JR- 503. During the course of the study, it was found that biofertilizers and different varieties significantly affected plant height, number of leaves per plant, number of tillers per hill, length of panicle, number of grains per panicle, number of filled grain per panicle, test weight, grain & stover yield of rice. Higher plant height (80.93 cm), number of leaves per plant (57.20), number of tillers per hill (20.87) at maximum crop growth stage of 90 DAT were recorded under the application of Azospirillum biofertilizer @ 250 ml/ha with rice cultivar of IR-64. Similarly, resulted in highest length of panicle (26.63 cm), number of grains per panicle (167.60), number of filled grain per panicle (153.07), test weight (22.19 g), grain yield/ha (61.46 q/ha) and stover yield/ha (106.68 q/ha) recorded under same treatment combination of Azospirillum biofertilizer @ 250 ml/ha with rice cultivar of IR-64. It was concluded from the results that application of Azospirillum biofertilizer @ 250 ml/ha with rice cultivar of IR-64 improved yield and yield components of rice.

 Keywords: Biofertilizers, Varieties, Azospirillum, Panicle, Test weight, Stover yield.