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The occurrence of biofilm forming Aeromonas spp. in drinking water in the Tiruchengode area was
monitored. Aeromonads cause traveler’s diarrhea in millions of peoples; particularly traveler’s visiting
less developed regions in Asia. The main source of infection is contaminated water.A total of 100 water
samples were collected from several sampling sites during a period of one year. Among these only 50
isolates were found to be positive for Aeromonas spp. The Starch-Ampicillin agar were used as a
selective presumptive isolation medium for the isolation of bacterial isolates from the samples and
confirmed as Aeromonas hydrophila 30(60%), Aeromonas caviae 20(40%) were determined by using
standard biochemical analysis according to Bergey’s manual of systematic Bacteriology. Susceptibility
tests were carried out according to the criteria of national committee for clinical laboratory standards.
MIC’s were obtained by agar diffusion method. Slime/Biofilm producing isolates were studied on Congo
Red Agar (CRA) method. The detection of the presences of such virulence factors is a better indicator of
the potential health rish, Infection due to bacterial pathogen with such virulent factors through contact
with water (or) infected person act as a health hazards to humans. So that, In our study slime/biofilm
forming isolates were detected and inactivated by using Silver nitrate. The samples taken from the
drinking water in Tiruchengode area were found to contain very diverse biofilm forming Aeromonas
populations that can pose a risk for the residents of the city.
Key Words: Aeromonas spp., Antibiotic sensitivity test, Biofilm, Antibactericital activity of Silver nitrate.