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The present investigation on response of organic manures and bio fertilizers on floral characters in tuberose was conducted at Model Floriculture Centre, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, during 2010 to 2012. The experiment was laid out in Randomized Block Design with four replications. The treatment was comprised of Organic Manures and Bio Fertilizers (Poultry manure, vermicompost, Trichoderma harzianum and Pseudomonas fluoroscens). Trichoderma harzianum (40 g/ m²) resulted in maximum diameter of lowest floret in pooled value (4.27) while the minimum diameter of lowest floret in pooled value was observed in treatment poultry manure (1 kg/ m²) (2.51). Pooled value recorded maximum rachis length (23.24 cm) in treatment vermicomost (2 kg/ m²) whereas, treatment poultry manure (1 kg/ m²) recorded minimum rachis length (17.46 cm). Pooled performance of both the years showed that treatment poultry manure (0.5 kg/ m²) attained maximum total number of florets per spike (39.06) and it was minimum in treatment poultry manure (1 kg/ m²) (31.33). The pooled data for both the years of study showed that maximum number of opened florets per spike was recorded in treatment Trichoderma harzianum (20 g/ m²) (25.64) and minimum  number of opened florets per spike was observed in treatment vermicompost (2 kg/ m²) (22.61). Mean performance for minimum number of unopened florets per spike was recorded in poultry manure (0.5 kg/ m²) (8.72) treated plants and maximum in untreated control (14.16) plants. The pooled value of yield of spikes per square meter showed the maximum value (66.25) for treatment vermicomost (2 kg/ m²).

 Keywords: Trichoderma harzianum, Vermicomost, Pseudomonas fluoroscens,