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A field experiment was conducted to evaluate the effect of Sulphur levels and varieties on growth, yield and quality of soybean. Experiment comprised 12 treatment combination involving four soybean varieties (JS- 2034, JS- 2029, JS- 2068 and RVS-2001-04) and three Sulphur levels (0, 25 and 35 kg/ha) treatment in randomized block design with factorial concept replicated thsoybean during kharif season of 2020-21 at Instructional Farm of Department of Agronomy, Faculty of Agriculture, AKS University, Sherganj, Satna (M.P.). Statistical analysis of the results revealed that individually soybean variety of RVS-2001-04 and sulfur application at 35 kg S/ ha reported to have maximum plant height (46.78 cm), number of branches per plant (9.00) at maximum crop growth stage, number of pods per plant (33.53), length of pod (4.88 cm), number of seeds per pod (3.73), seed index (10.57 g), seed (16.67 q/ha) & straw yield per hectare (33.84 q/ha) and oil content (21.60 %). It was concluded from the results that application of Sulphur @ 35 kg/ha for soybean variety of RVS-2001-04 improved yield and yield components of soybean. Keywords: Soybean, Sulphur, Test weight, Pod, Seed index, Straw yield.