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For pearl millet production in the semi-arid tropics, Sclerospora graminicola is a major biotic constraint causes Downy mildew. The pathogen is heterothallic and frequent recombination leads to evolution of new virulent populations. Identification of resistance to new virulent isolates is a prerequisite for resistance breeding. A very good understanding of ability to manipulate oligogenic and quantitative traits is offered to plant breeders by recent advances in genetic marker technology. In the present investigation, Genotypic data generated for a total of 39 marker loci (SSRs) were used to construct a linkage map of the pearl millet mapping population of 295 Recombinant Inbred Population based on the cross 81B-P13 × AIMP 92901-deriv-P03.A skeleton linkage map of seven linkage groups with a total map length of 536.8cMwasconstructed using Map Maker/Exp version 3.0b at LOD threshold value of 3.0 and map was drawn using Map Chart 2.2. Among all the linkage groups of the present study, linkage group1 has the highest map length (146.6 cM) followed by linkage group 2 (98.3 cM). The linkage group 3 (6.6 cM) has been recorded as the shortest among all seven linkage pearl millet groups in this study.

Key words: SSR Markers, Linkage group, Population, Genotypic