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Twenty five rhizospheric soil samples were collected from five different sampling sites and a total of 29 phosphate sollubilizing Bacillus isolates were obtained. From primary and secondary screening of these isolates, best 5 phosphate sollubilzers SST3, SST4, SLYV3, SDR1, SDR2 were found to produce 15 μg/ml, 11 μg/ml, 13 μg/ml, 18 μg/ml and 12 μg/ml solubilised phosphate respectively. These isolates were further selected for preparing compatible consortia for on field application. Along with phosphate sollubilization, isolates SST3, SST4, SLYV3, SDR1 and SDR2 showed considerable PGPR activities like 54 μg/ml IAA, 50 μg/ml, 40 μg/ml IAA, 36 μg/ml IAA and 34 μg/ml IAA production; 1.2 μg/ml, 0.79 μg/ml, 0.83 μg/ml, 1.01 μg/ml and 1.35μg/ml protease production. All the five isolates were found positive for siderophore production, N2 fixation, cellulase production, HCN production while isolate SST3 showed antifungal activity against fungal pathogen Colletotrichum falcatum. From 16S rRNA sequencing SST3, SST4, SLYV3, SDR1 and SDR2 isolates were identified as Bacillus cereus, Bacillus pumilus, Bacillus cereus Bacillus subterraneus, Bacillus cereus respectively.
Keywords: Bacillus, Phosphate sollubilization, Plant Growth Promoting Rhizobactera, Bio fertilizer