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Sexual dimorphism is a characteristic phenomenon exhibited by Libellulidae and Aeshnidae family
of Odonates. Diplacodes trivalis is a sexually dimorphic Libellulidae species and the present study
was carried out to check whether any genetic change had occurred in both the sexes and how it is
phylogenetically related with other Odonate members. DNA barcoding using CO I gene offers the
opportunity for a standard system of species identification based on the analysis of small fragment of
DNA. The PCR amplification of partial cytochrome oxidase I gene of Diplacodes trivalis yielded a
product of 466bp length (GenBank Accession: KP 835512).The length and nucleotide sequence of
DNA and was found to be similar in both sexes. BLASTn program showed 99% sequence similarity
to the same species reported from Mizoram, TamilNadu and Japan. The result indicated that this
sexually dimorphic species does not have any genetic changes with respect to their morphological
differentiation.
Key words: Odonates, Diplacodes trivalis, DNA barcoding, Cytochrome oxidase I gene