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Silver nanoparticles are one of kind of nanomaterials with applications in areas like cosmetics, nanosensors, antimicrobials and bioremediation. In modern era, ecofriendly processes of silver nanoparticles synthesis using various biological systems are gaining importance, biosynthesis of silver nanoparticles using bacteria is one of such approach with benefits of easy maintenance and manipulation. Optimization of culture conditions is useful for harvesting maximum product benefit from microorganisms. Present study focuses on biosynthesis of silver nanoparticles using Bacillus siralis strain UMBS1.1 followed by optimization of its culture conditions using response surface methodology. By using a central composite design, the optimal culture conditions for silver nanoparticles synthesis were found to be as: incubation time 46.0 hrs; incubation temperature of 36.5°C; pH 8.5, 3.0 g/l tryptone and 3.0 g/l yeast extract. Under these conditions, a 16.064 fold increase in silver nanoparticles activity has been achieved as compared to unoptimized conditions. These silver nanoparticles were found to show antimicrobial activity against various fungal phytopathogens.

Key words: Bacillus siralis strain UMBS1.1, Silver nanoparticles, Response surface methodology, Antifungal effect