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Senna obtusifolia (L.) Irwin and Barneby of the tribe Cassinae under family Leguminosae is growing in wild in different parts of India and abroad. Rhein, an anthraquinone, is a biochemical of multifarious medicinal properties like, correction of maladies of liver, kidney, anti-oxidant, anti-obesity and even anti-cancer. The presence of this anthraquinone from the leaves of the species has been reported in the present paper. The extraction process through hydrolysis of sennosides yielded a mixture of anthraquinones from which the rhein was isolated. With the preliminary assurance of the chemical through Thin-layer chromatography quantification of the chemical was done by high-performance-liquid chromatography.

Key words: Senna obtusifolia, Rhein, Sennosides, Thin-layer chromatography, High performance liquid chromatography.