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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2013 , Volume 1, Issue 1
Page No. : 1-6
Article doi: : http://dx.doi.org/10.18782/

<p>An improved method for callus culture and In vitro propagation of garlic (Allium sativum L.)</p>

Jitendra Mehta*, Arti Sharma, Neha Sharma, Surendra Megwal, Gajendra Sharma, Priyanka Gehlot and Ritu Naruka

Plant Tissue Culture Laboratory and Department of Biotechnology, Vital Biotech Research Institute, University of Kota, Kota- 324009 (Rajasthan), India

 ABSTRACT

This review highlights the recent development and achievements made for the multiple shoots regeneration and callus induction of Allium sativum. Shootlets were regenerated from nodal explants of stem through auxiliary shoot proliferation. The inductions of multiple shoots from nodal segments were highest in MS medium supplemented with 1.0 mg/l Kn. For rooting different concentration of IBA were used and highest rooting was recorded on MS medium supplemented with 2.0 mg/l IBA. The rooted Plantlets were hardened initially in culture room conditions and then transferred to misthouse. Leaf petiole explants were used for the purpose of callus induction. Best growth was observed in MS medium supplemented with 0.25 mg/l 2, 4-D+ 0.5 mg/l Kn and 0.25 mg/l 2,4-D+ 0.5mg/l BAP.

Key Words:- Allium sativum , Shoot proliferation, Callus, Garlic.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/

Cite this article:,

Int. J. Pure App. Biosci. 1 (1): 1-6 (2013)