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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2017 , Volume 5, Issue 2
Page No. : 1199-1206
Article doi: : http://dx.doi.org/10.18782/2320-7051.2430
Horizontal Transfer of Heavy Metal Resistance Plasmid from A Brahcish Waster Bacterium Pseudomonas Sp. AMET1221 to Escherichia coli DH5ἀ
M. Jayaprakashvel1, C. Divya Bharathi2, R. Muthezhilan3 and A. Jaffar Hussain1*
1Department of Marine Biotechnology, AMET Deemed to be University, Chennai, Tamil Nadu
2Department of Biotechnology, Kamaraj College of Engineering & Technology, S.P.G.C. Nagar, Virudhunagar, Tamil Nadu
3Department of Biotechnology, Dwaraka Doss Goverdhan Doss Vaishnav College, Chennai, Tamil Nadu
*Corresponding Author E-mail: jaffar.a@ametuniv.ac.in
Received: 29.12.2016 | Revised: 10.01.2017 | Accepted: 12.01.2017
ABSTRACT
A total of forty eight heavy metal resistant bacterial strains were isolated from collected water samples of Kanathur Beach and Buckingham Canal at Kanathur, TamilNadu, India. Based on the minimum inhibitory concentration (MIC) of the strain toward chromium and mercury, resistance was found to be 50μM and 10mM respectively. MIC determinations amongst four strains (AMET1217, AMET1221, AMET1229 and AMET1242) were screened as highest heavy metal resistance bacteria. The present study proved the genetic contribution of heavy metal resistance in this strain to be plasmid mediated. Plasmid curing experiments affirmed plasmid mediated heavy metal resistance. Additionally, genetic transformation of a non metal resistant lab strain Escherichia coli and the cured strain of Pseudomonas sp. AMET1221with the isolated plasmid increased their metal tolerance level and confirming the genetic determinant to be present in the plasmid. The metal-resistance properties of these isolates are possible biotechnological tools in heavy metal bioremediation.
Keywords: Biotransformation, Environmental Pollution, Metal Resistance, Plasmid curing
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2430
Cite this article:
Jayaprakashvel, M., Bharathi, C.D., Muthezhilan, R. and Hussain, A.J., Horizontal Transfer of Heavy Metal Resistance Plasmid from A Brahcish Waster Bacterium Pseudomonas Sp. AMET1221 to Escherichia coli DH5ἀ, Int. J. Pure App. Biosci. 5(2): 1199-1206 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2430
