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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2017 , Volume 5, Issue 3
Page No. : 443-448
Article doi: : http://dx.doi.org/10.18782/2320-7051.2552
Phylogenetic Analysis of the Bacteria Associated with the Fresh Water Micro-Alga Chlorella sp.
R. C. Patil* and B. L. Jadhav
Department of Microbiology, Bhavan’s College, Andheri (W), Mumbai- 400058
Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai- 400098
*Corresponding Author E-mail: rcpatil68@gmail.com
Received: 5.02.2017 | Revised: 16.02.2017 | Accepted: 18.02.2017
ABSTRACT
The importance of the microbial association with fresh water algae is well known and reported by several researchers. However, majority of these studies focus on the interaction between the algae and the microbes and there are very few reports on the phylogenetic studies on these associated microbes. Therefore, in the present investigation, molecular phylogeny of the three different bacterial species associated with the Chlorella sp. was determined by amplifying and sequencing the genomic 16S rRNA region. The DNA sequences were analyzed using online BLASTn tool. The BLAST results were used to find out evolutionary relationship between these bacteria. The phylogenetic tree for these bacteria was constructed by using MEGA 5 software.
Keywords: Polymerase chain reaction, 16S rRNA, Phylogenetic tree, Evolutionary relationship
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2552
Cite this article:
Patil, R.C. and Jadhav, B.L., Phylogenetic Analysis of the Bacteria Associated with the Fresh Water Micro-Alga Chlorella sp., Int. J. Pure App. Biosci. 5(3): 443-448 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2552
