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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2017 , Volume 5, Issue 4
Page No. : 698-701
Article doi: : http://dx.doi.org/10.18782/2320-7051.3021

RT-PCR Based Diagnosis of Soybean Mosaic Virus

H. V. Nandakishor¹, B. Kumaraswamy^2*, S. S. Mane³ and G. Amrutha Veena⁴

Department of Plant Pathology, Post Graduate Institute, Dr. PDKV, Akola, 444104 Maharashtra, India
*Corresponding Author E-mail: nandakishorehv@gmail.com
Received: 25.05.2017 | Revised: 7.06.2017 | Accepted: 8.06.2017

ABSTRACT

The occurrence of Soybean mosaic virus (SMV) in soybean was confirmed by symptomatology, reverse transcription-polymerase chain reaction (RT-PCR). SMV on soybean cv. JS-335 produced characteristic symptoms such as dark green colour of leaves, mosaic and mottling symptoms, crinkling, leaf puckering of leaves. The soybean mosaic virus was detected and diagnosed by applying the RT-PCR technique. The RT- PCR technique was performed using a specific primer pair CIF/CIRev and POT1/POT2. RT- PCR technique revealed presence of SMV, with a band length of 700 bp and 725 bp have been obtained from SMV infected samples.
Key words: Soybean, Soybean mosaic virus, RT-PCR.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.3021

Cite this article:

Nandakishor, H.V., Kumaraswamy, B., Mane, S.S. and Veena, G.A., RT-PCR Based Diagnosis of Soybean Mosaic Virus, Int. J. Pure App. Biosci. 5(4): 698-701 (2017). doi: http://dx.doi.org/10.18782/2320-7051.3021

No. 772, Basant Vihar, Kota

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