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In this study focus on shelf life of different carrier based bioinoculants for this Pseudomonas cultures collected from different resource labs, then cultures were studied morphologically and biochemically for purity confirmation. The screening test results revealed that, among all Pseudomonas isolates, (RGP-1) showed best plant growth promoting abilities in in-vitro conditions. The selected Pseudomonas (RGP-1) isolate was multiplied in large quantities in appropriate culture broth by incubating at 28 ± 2°C in an incubator shaker till they attained log phase with a cell load of l × l09cfu ml-1. For biofertilizer production Vermicompost, Vermiculate, Lignite, and Sodium Alginate carriers were used for bioinoculant preparation and stored at different temperatures i.e. 4ºC and 28± 2°C. In first month log10 values are 9.62, 9.82 (in Vermicompost), 9.73, 9.84 (Sodium Alginate), 9.50 9.75 ( Lignite), 9.70, 9.82 (Vermiculate) showed at 4ºC and 28± 2°C respectively, Even though bioinoculant populations were higher at first month gradually deceasing upto 8th month, at the end of the experiment log10 values are 8.20, 6.50 (in Vermicompost), 8.90, 7.20 (Sodium Alginate), 7.80, 6.00 (Lignite), 8.50, 6.80 (Vermiculate) showed at 4ºC and 28± 2°C respectively. Finally concluded that Sodium Alginate is best carrier materials among them when compared to specification of biofertilizers, carrier should be minimum 5×107cfu g-1(7.6 log10) viable count of powdered form of carrier based biofertilizer at both storage temperatures.
Keywords: Pseudomonas, carriers, Vermiculite Vermicompost, Sodium alginate, temperatures. Research Article Cite this